The synthesis of RNA, including the initiation and elongation of RNA transcripts, involves the utilization of adenosine triphosphate (ATP) as an essential energy source and activator.

1. ATP's Role in Transcription Initiation: ATP plays a crucial role in the initiation of transcription from promoters by RNA polymerase. It is involved in the synthesis of accurately initiated transcripts from specific promoters, such as the adenovirus 2 major late synthesis 80532-66-7 and mouse interleukin-3 promoters. The synthesis of run-off transcripts and transcription intermediates requires ATP for both RNA synthesis and activation of the transcription system prior to RNA synthesis

2. Activation of RNA Polymerase II Transcription Complexes: ATP-mediated activation of RNA polymerase II transcription complexes is a critical step in RNA synthesis. The addition of ATP and other nucleotides initiates RNA synthesis, and the presence of ATP analogs affects the activation of transcription complexes

3. ATP Requirement for RNA Polymerase: While the statement "RNA polymerase requires ATP to make RNA" emphasizes the fundamental role of ATP in RNA synthesis, it also highlights the complexity of the ATP-dependent processes involved in transcription

4. ATP in RNA Synthesis and Processing: ATP has multiple roles in the synthesis and processing of mRNA. It is involved in the initiation and elongation of RNA transcripts, as well as in the termination and cleavage of nascent RNA